Practice English Speaking&Listening with: Lecture - 13 Lipids and Membranes 1

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Lipids are non-polar hydrophobic compounds that have a polar head group and a hydrophobic

tail. Now we are going to understand how these are organized into membranes and what their

functions are. So basically anything that is a non-polar hydrophobic compound soluble

in organic solvents is called a lipid.

The membrane lipids that we talk about are called amphipathic in nature. Amphipathic

means it has both a non-polar end and a polar end to it. The non-polar end arises due to

definite chemical moieties, definite groups and polar end again arises due to some other

groups that at are present. If we look at the functions of lipids they play a very important

role in the biological cells and biological cell membranes and of course membrane transport

which will be doing later on. We have steroid hormones, in digestion, as fats and triacylglycerols

which give us the fuel for our bodies and the membrane structure. So lipids are involved

in all of these activities starting from hormones to digestion elements that are present in

the bile. These are all lipids and their components. The fats and triacylglycerols are lipids and

in the membrane structures, which is what we will be doing today, we have fatty acids,

phospholipids, sphingolipids and cholesterol.

Initially we are going to study the different nomenclature and the different types of fatty

acids. A fatty acid is a long chain acid. In the organic carboxylic group that we speak

about we speak of aC=OOH being the acid moiety. Here we have a long chain hydrocarbon

with a -COOH attached to it.

These fatty acids are sigma-bonded carbon chains. Sigma bond mean we have just single

bonds here and they have at the end a carboxylic acid moiety, a carboxylic acid functional

group. This functional group or this moiety is going to be used in the overall function

or overall structure of the lipids that form these membranes. If we consider the fatty

acids, the first thing that we are going to look into is their structure and the nomenclature

as to how they are identified and then the physical and the chemical properties of the

fatty acids. Then we will study about the triacylglycerols and see how they play an

important role in the structure and function and the formation of lipid membranes. The

first thing is about the nomenclature. The nomenclature of the fatty acids actually follows

two types.

One is called the n-designation and other is called the delta designation. In the n-designation

you can straight away see that the numbering is from the extreme end away from the carboxylic

acid group. In this case the numbering is from here, 1, 2, 3 and 4. For the delta designation

the numbering begins from the carboxylic acid end and it is this nomenclature of this designation

that we will be using the delta designation. If you are to write the structure of any particular

fatty acid there will be a specific nomenclature that you will follow and that nomenclature

will be the delta designation where the numbering will begin from the carboxylic acid end, not

the n-designation. How does this help us?

In the next slide we will see as to how we can write the nomenclature. This is a fatty

acid in the n-designation and what I have below here is a fatty acid in the delta designation.

From this nomenclature you should be able to write the fatty acid. We have to know what

each of these numbers mean. The first number that you have here is the carbon chain length.

It tells you how many carbon atoms you have. After the colon you see another number. This

20 means that the carbon chain length is 20. The number after the colon designates the

number of double bonds present. In this case we have carbon chain length of 20 and the

number of double bonds is 4. Now you can tell me that this is actually the position of the

double bonds. So the number that we have at the end is the position of the double bonds.

The delta 5 means the double bond is between 5 and 6, 8 means it is between 8 and 9, 11

means between 11 and 12 and 14 means between 14 and 15. In the n-designation there is usually

just one number put because usually when we form or when the fatty acids are biosynthesized

what happens is they form in specific units. If you notice here every double bond is after

three carbon atoms. It is 5, 8, 11 and 14.

In the n-designation, only one is specified which tells you that there is going to be

another one at 9, 12 and 15 and it is opposite to this because this numbering is opposite

to that in the delta designation. The double bonds are assumed to be spaced

by three carbons. So here in the n-designation only end 6 is specified and nothing else.

But in the delta designation the position of the double bond is written explicitly where

we now know that if this is the nomenclature it means that if you have a carbon chain length

of 20 there are 4 double bonds and the position of the double bonds are 5 and 6, 8 and 9,

11 and 12 and 14 and 15. If we look at the set of designations, another thing that we

should mention here is that the double that we see in the fatty acid usually have a cis

configuration and most naturally occurring fatty acids have an even number of carbon

atoms because the way that they are biosynthesized they come in pairs of carbon atoms. If they

come in pairs of carbon atoms all of these are usually even numbers. You do not see an

odd numbered fatty acid because when fatty acids bio synthesis occurs it comes in pairs

of carbon atoms.

If we look at some fatty acid and their common names 14:0 is myristic acid. These are all

the delta configurations. So you should be able to write what myristic acid is. What

is it? Its just a long chain, a hydrocarbon chain with 14 carbon atoms and you do not

need numbering in this case because there are no double bonds.

So we have myristic acid, palmitic acid, stearic acid, then we go to oleic acid. It is 18:1

cis. The cis is not usually put in, just delta nine is sufficient because most of them are

cis any way. So 18:1delta 9 means that oleic acid is an 18 carbon fatty acid with 1 double

bond between 9 and 10. That is as simple as that. The one that I had on the previous page

is actually arachidonic acid. It was 20 with 4 double bonds at 5, 8, 11 and 14. Eicosapentanoic

acid which is an omega three fatty acid has an additional double bond at position 17.

So this nomenclature is sufficient to tell you how to write a fatty acid. This is basically

the nomenclature of fatty acids and we are going to see how we can use these fatty acids

in forming our lipids.

What happens if you have this cis double bond? Here we have a single cis double bond. You

see how the carbon chain has now changed direction. If this cis or if this double bond did not

exist it would have been a nice straight chain and they could have been rotations about the

single bond. But when we have it in the cis configuration then what happens is there is

a break in the chain because of the cis configuration. You have what is called a kink in the chain.

Instead of having a normal long chain that we would have had and free rotation about

the single bond each cis double bond causes a kink in the chain. If I had another cis

double bond at this position this part of the fatty acid or this part of the chain would

fold back. So I would have a kink in the structure. So I have a kink in the structure due to the

fact that I have double bonds in the hydrocarbon chains. When we have these membrane lipids,

this is something we are going to study in detail later on, these are my fatty acid chains.

I am going to have a polar head group, we will see what those polar head groups can

be, and my hydrophobic tail if it is a straight chain fatty acid it will look like this. If

it happens to have one fatty acid that has a kink to it, it is going to be shaped like

this. We will see how I am talking about two fatty acids linked to a single polar head

group in a moment. But when we are talking about the polar head group and different fatty

acids when they link together you see how you can change the structure of the lipids

because of the type of fatty acids that is being attached to the polar head group. You

are basically changing the structure depending on the choice or the type of the fatty acids

that you are considering.

Basically if you look at the fatty acids, this would be the structure where we would

have carboxylic group here. This would be the polar part of it and if we have a long

chain it would be a smooth long chain, a straight chain. If you happen to have a cis bond here

what would happen? The chain would get bend.

You would have what is called a kink and you recognize that if you had another cis bond

here it would twist even more. Here are some physical and chemical properties of fatty acids. Fatty acids are weakly acidic

in nature; weakly acidic with a pKa of 4.5 to 5 which means that they are ionized at

physiological pH. The physiological pH is 6.7.4.

Saturated fatty acids are solids at room temperature. The melting point is going to depend on the

chain length and definitely the number of double bonds present; on the degree of unsaturation

and we will see how that is going to play an important part in our lipid formation,

membrane lipids. So we have weakly acidic fatty acids. The saturated fatty acids are

solid at room temperature. The melting point depends up on the chain length and the degree

of unsaturation. With the kink in the cis bonds what happens is it disrupts the molecular

packing. So it lowers the melting points. If you had straight chain that would normally

completely very well organize, you would have a higher melting point. But due to the presence

of the cis double bond the intermolecular packing of the hydrophobic chains is disrupted.

It is broken and that lowers the melting points. The polyunsaturated fatty acids that you see

in a lot of vegetables oils that you consume they say that they are pufa. That is what

it is called polyunsaturated fatty acids; they are readily oxidized by exposure to air

and these fatty acids can form micelles. You know why they can form micelles? Because they

have a hydrocarbon chain and it has a polar head group to it. These are the basic physical

and chemical properties of fatty acids. What we have to remember is that they are weakly

acids, saturated fatty acids are usually solid at room temperature and the melting point

is going to depend up on the number of carbon atoms you have and on the degree of unsaturation

and the more the number of cis bonds that you have the lower the melting point is going

to be because you are going to disrupt the intermolecular packing between the hydrophobic

chains.Now we are going to come to what are called Glycerophospholipids.

We have glycerol. Glycerol is CH2OH CHOH-CH2OH. Glycerophospolipids are what comprise lipid

membranes. They form or they are the constituents of cellular membranes. You recognize that

these -OH groups that you have here can be esterified by acids. What is an esterfication

reaction? We have ROH and RCOOH. With the removal of water, we form a -OCO an ester

formation. That means that these Hs if they react with fatty acids can be esterified

and I can have to this glycerol a long chain attached to either this hydrogen or this hydrogen

or this hydrogen. Usually there are two fatty acids attached to it which is why I have two

lines sticking out from the polar head groups. We have the hydroxyls at C1 and C2 that are

esterified with the fatty acids. We have our glycerol. The one that I show you here is

triacylglycerols which is what comprises the fat droplets that we have in cells.

If you see there are triacylglycerols or triglycerides test that have to be performed in blood to

see whether you have appropriate triacylglyceride content. If you have more fat droplets then

you have fat restricted diet. This is what a triacylglycerol would look like. Here is

the structure of glycerol. We have three -OH groups here. If each of them number 1, number

2 and number 3 are each esterified, this is what it is going to look like. So in the first

carbon atom and in the third carbon atom we have straight chain fatty acids that have

been used to esterify the -OH groups of the glycerol. The extreme -OH groups of the glycerol

have been esterified with straight chain fatty acids here. In the middle we straight away

know that this has now not only one but it has two cis double bonds which is why it is

even bent further than the one I showed you previously. If you look at the structure here

this is linolinic acid that has been used. We have one cis bond here and another cis

bond here. So it has changed the structure of the hydrocarbon chain into making it more

disrupted. Its more kinked in a sense.

We are going to see how we can change the properties of the groups here and then see

what the lipids or the glycerophospholipids are actually made of. What we have are called

phospholipids. What are these phospholipids? In the two classes of phospholipids that are

present these form cell membranes. We have glycerolphospholipids that have a glycerol

backbone just like I showed you. We have sphingomyelin that forms from a spingosine backbone and

in this case this actually forms a lot of the membranes and these phospholipids are

usually refered to as PL.

What is essential of these? They are extremely important for membrane structure. They are

found in membrane lipids. We will see what these structures actually are. What we need

to know is there are two types of phospholipids and they are essential for the membrane structure

and they are found in membrane lipids. This is the break up. What we have here is we have

storage lipids. Storage lipids like storage in terms of fat droplets.

What are the fat droplets? They are triacyl glycerols. We need to know is that the storage

lipids which are neutral in nature have three fatty acids attached to the glycerol. We have

membrane lipids. In the membrane lipids we have phospholipids and glycolipids. In phospholipids

we can have glycerophospholipids or sphingolipids. It is just a break up tree. The membrane lipids

are polar in nature because they have a phosphate group attached we will see what that means

in a minute. Phospholipids are glycerophospholipids or sphingolipids and glycolipids are other

sphingolipids. Glyco means you have sugar. Whenever the word glyco comes in a prefix,

glyco means there is sugar present. If we go back to the phospholipids break up we have

storage lipids that are triacylglycerols fatty acids. We have membrane lipids that are phospholipids

or glycolipids. The breakup of phospholipids is glycerolphospholipids or sphingolipids

where the backbone basically different.

Now we will study this in a bit more detail. The black part here is part of glycerol CH2OH

CHOH CH2OH. What has happened at the first two carbons is the C1 and the C2 have been

esterified by fatty acids.

We have long chain fatty acids in both cases. The third carbon has been esterified with

the phosphate. Remember that is also an acid. We have two of the carbons esterified with

fatty acid chains and one with the phosphate. This is called a phosphatidate. What is the

basic structure? The basic structure is glycerol. The two carbons of glycerol have been esterified

with two fatty acids and the third with phosphate. We have a glysphosphatidate or otherwise glycerophospholipids.

This is what it looks like. We have a fatty acid on the first carbon, we have a fatty

acid on the second carbon and we have a phosphate on the third carbon. The phosphate again is

esterified. If the phosphate again is esterfied by this X group this is where we can change

the type of glycerophospholipids that we have. So where can we make the changes? We have

the basic structure of glycerophospholipids that is going to be the glycerol.

We have one fatty acid linked on the first carbon, a second carbon linking another fatty

acid. So we can change the type of fatty acids that we have. As soon as I change the type

of fatty acid the type of lipid is going to change. Then I have an esterification on the

third carbon atom with phosphate and I have a polar head group here and we will see how

that affects it. Where is my polar head group? It is here. Here are all the oxygens and the

phosphates and where are my chains? R1and R2. I have the basic structure of the lipid

that is going to look like a polar head, that is this part here, and the R1 and the R2 are

these chains. You understand what the structure looks like now? We have the overall glycerol,

two of theOHs have been esterified with long chain fatty acids which is why I

have two legs to this polar head group and the polar head group forms because of the

phosphate esterfication and an additional polar head group. This is the basic structure

of a glycerophospholipid. What can I change here? I can change type R1. I can change R2,

I can change X. In that I will be changing the complete type of glycerophospholipid that

I have and we are going to see how we can do that.

This is our structure. So we have the Pi-OH. What is happening to thisOH? It has been

esterified again. It can be esterified with serine, choline, ethanolamine, glycerol or

inositol. There are different groups that can be used to esterify the phosphate in the

phospoglyceryl and the two fatty acids that we have R1 and R2 are usually not the same.

We will see why later.

How can they be different? They can be different in their length, they can be different in

the number of double bonds and they can be different in the location of these double

bonds. That is where we have a difference R1, R2, X. So what are the differences? This

is phosphatidylinositol.

You recognize now the glycerol moiety. Here is a glycerol moiety. So this is one carbon

atom, this is the other carbon atom and this is the third carbon atom. What was our glycerol

structure? -COH then we have CH-OH and again we have CH2OH. This has been esterified and

this has been esterified. We have long chains here and in this case we have it esterified

with the phosphate. Linked to the phosphate again now is another group. This is X that

I showed in the previous slide. So it is this group X that in this case is inositol. When

you have all this number of OH here and the phosphate here and the negative charge here

what does this comprise? It comprises the polar head groups. It is this part that forms

the polar head groups and what is R1 and R2 forming? The tail. It can be different depending

on the type of R1 and the type R2.

This is the basic structure of a glycerophospholipid. Now what can we change? We can change inositol,

make it something else. Let us see what we can change it to. We can make it choline.

Again the basic structure is exactly the same. I have R1, R2. I have the phosphate. Linked

to the phosphate I have another X. What is that X? Choline. So I have instead of phosphatidylinositol

I have phosphatidylcholine. I can also have phosphatidylethanolamine. I can have phosphatidylserine.

In each of these the difference is going to be just in the X group in this case. We can

have identical R1s and R2s for phosphatidylchloine, phosphatidylserine and phosphatidylethanolamine.

These are the different types of glycerophospholipids that we can have. What you need to remember

is the basic structure is a glycerol, you have two fatty acids R1, R2, you have a phosphate

and the phosphate is linked again to another polar head group that is going to result in

a polar head group to your lipid. What is a sphingolipid? This is the structure. It

is based on the structure called sphingosine.

This is the structure of spingosine. You have a -CH2OH. You have in the middle CH-NH3+ and

in the last carbon you have aCH-OH to which is linked a long hydrocarbon tail. This

is the basic structure of sphingosine. We have a long carbon chain as it is. By default

sphingosine comes with a long hydrocarbon chain. It has a polar region here that constitutes

an amino group. It has a NH3+. What can happen with a NH3+ and a fatty acid? It can form

an amide. In the part here we have an RC=O-NH. Just like an amino acid linked to NH3+would

give you an amide, you can have an amide formed here. The amino group of the sphingosine that

can form an amide bond with the fatty acid gives you what is called a ceramide. Have

you heard the word ceramide before? They sell you shampoos with ceramide in it. If you look

at the advertisement of shampoos they will tell you that ceramides are present in it.

This is what a ceramide is. What do you have in a ceramide? You basically have a sphingosine.

What is a sphingosine? Sphingolipids are going to be derivatives of this. It has nothing

but a long hydrocarbon chain, there is NH3+ attached to it and an -OH attached to it.

This already has the long hydrocarbon chain attached to it. You can have this NH3+ form

an amide with another fatty acid. You are going to have two long carbon chains here.

We have ceramides that usually include a polar head group and they are esterified to the

terminal -OH of the sphingosine. We will see what it is. You recognize the basic structure

of the sphingosine now? This is the basic structure of the sphingosine, the one in black.

This is the long carbon chain. This is the sphingosine moiety. This is the fatty acid attached to it to form

an amide and we had an -OH here. So this can attach to another group forming a sphingomyelin.

We have what is called a sphingomyelin which is a ceramide with the phosphocholine. What

is a ceramide? A ceramide is when you have -OH here and the amide here and the sphingosine

as it is. So in the basic structure of the sphingosine if you have the fatty acid linked

to form an amide it is called a ceramide. In this case when you have phosphocholine

to form a head group here this forms what is called a sphingomyelin. So this is after

the formation of a ceramide. So you have an -OH group, initially this was anOH. This

is now linked to phosphocholine to form what is called a sphingomyelin. There is another

thing that we need to know. We remember what a sphingomyelin or what a ceramide is? What

is a ceramide? A sphingosine with the fatty acid withOH.

Now you have to recognize that that H can be replaced. If you replace this H with phosphocholine

you have sphingomyelin. If you replace it with a sugar you have what is called a cerebroside.

Its just nomenclature. All you need to know is you have a glycerol, you have a sphingosine.

These are the two backbones. Thats it. You have three -OH groups here. If three of them are fatty

acids you have a triglyceride. If two of them are fatty acids and one of phosphate you have

glycerophospholipids. If that phosphate again is attached with another X, you have series

of lipids. In sphingosine you have different types. Basically you have a shape like that.

You have a long carbon chain in the structure of sphingosine itself. You have fatty acid

attached in forming a ceramide. Then this -OH can be linked to sugar, to form a cerebroside.

It can be linked to phosphocholine forming a sphingomyelin. That is the basic structure

of all these. So we can have a cerebroside if you had just simple sugar or you can have

ganglioside when you have complex oligosaccharide attached to it. Its just the nomenclature.

They are usually found in the membrane bilayer which is why we have to consider all the different

types of possibilities of the sphingolipids of the lipid themselves. If we look at the

molecular arrangements of the lipids you know that they can associate with water because

the cytosol of the cell itself is embedded in water, embedded in the blood, embedded

in the cytosol. The hydrophobic tails will never be in the cytosol.

We have to have what is called a bilayer. So it is this polar part that is going to

be outside the cell and this polar part that is going to be inside the cell. So we have

a bilayer that has polar head groups in either directions. So the amphipathic lipids in association

with water will form complexes in which the polar regions are in contact with water and

the hydrophobic regions are away from the water. So it is a very smart way of forming

the lipids, where you have a strong bilayer which is not going to allow everything in

and out of the cell but at the same time it is going to be extremely important in the

characterization of the lipid bilayer. There is another way we can organize this. How is

that? In a spherical manner, we form what are called spherical micelles. What are these

micelles?

These micelles have polar head groups outside and we have the hydrophobic tails inside.

We can also have what is called a reverse micelle, where you have the opposite of this.

If you put this spherical micelle with the polar head groups in an organic solvent it

is going to reverse and the polar head groups are now going to be in the center and the

hydrophobic tails are going to be facing the hydrophobic or the organic solvent. So we

have what we call the reverse micelles or the normal micelles. When we have a cerebroside

or a ganglioside, some structure like this form a micelle then we have a long fatty acid

chain at the R group. We have another long hydrocarbon chain in the sphingosine moiety

…. because of the sphingosine structure itself. When this forms a micelle we expect

this part to be the polar head groups on the surface and these two to be the legs of the

structure. The structure that we have here is basically going to be the bilayer with

two long chains. In case of a glycerophospholipid both of these are fatty acid chains. But in

case of a sphingolipid one part is the hydrocarbon chain that belongs to the sphingosine and

this is the ceramide, the amide part that has been linked with the amide of the sphingosine

to form an amide. So we have one fatty acid and one sphingosine hydrocarbon chain and

the same thing that we would have here. What are the structures that you can have? This

is a micelle.

A micelle is not two dimensional it is three dimensional. It actually looks like this.

This is just like half of it cut of. We have different types of micelle structures. All

these red balls that we see here, red spheres along the surface are all polar head groups

and all the chains that are inside are all the hydrophobic chains that we see. Consider

a liposome and the way transport of material occur in the body. If you have a micelle and

you have a polar ingredient or a polar substance that has to be transported, you understand

that it is not going to be possible for this particular micelle to transport it. Why? Because

it has a hydrophobic core to it and it would be possible to transfer a hydrophobic component

but if it happened to be a polar part it would be difficult to do so. So we have the formation

of liposomes. What happens here is you see there is a lipid bilayers sort of a thing

that forms the membrane and inside we have a polar center. The reason why I am telling

you this is this is used for a lot of drug delivery. When you have drug delivery or when

you are creating or making drugs you have to ensure that the drug is water soluble.

If you want it to interact with blood plasma you have to have one that is going to be easily

solubilized, which is a problem with drugs that they are not easily solubilized.

The transport of a lot of material takes place through these liposomes. You understand that

in the center here we can have any polar moiety. Any favorable ionic interaction that might

occur will hold the drug in this position and it will transfer it to where it has to

go. It will circulate in the blood and then be able to transfer itself. In the case of

a bilayer is we have the most stable configuration for amphipathic liquids. This is the possible

structure and this is usually used in transport but this is sort of a confined structure.

If we have a lipid bilayer then we have the polar groups forming a sheet on one end and

the polar group forming the sheet on the other end. We have a lipid bilayer and it is this

bilayer that is going to result in all of the transportation, all of the lipids structure,

all of the membrane structures that we will be seeing in the next class.

Basically what we have learnt is that we have our glycerol. In the glycerol we have storage

lipids. What are storage lipids? Storage lipids are those that are fat droplets. What are

these fat droplets? The fat droplets are triacylglycerols or triglycerides. What is the basic structure in this case? We have

our three carbon glycerol and we have threeOH. We have this replaced by a fatty acid, this replaced

by fatty acid, this esterfied to fatty acid and each of these esterified to fatty acids

is going to give our triacylglycerols. They can be esterified by a series of fatty acids

and we learnt that if we have such a nomenclature delta 5, 8, 11, 14 we know how we can write

this. We have normally a long carbon chain where we have the -COOH attached in a normal

fatty acid. When we have a nomenclature in the delta designation we have 18:4. The 18

stands for the number of carbon atoms, the 4 stands for the number of double bonds and

these are the positions of the double bonds from 5to 6, 8 to 9, 11 to 12 and 14 to 15.

Once we have these fatty acids we can have a cis configuration to the double bond. As

soon as we have this in a cis configuration this changes the direction. As soon as this

changes the direction then what happens is I have a kink. This gives rise to a kink in

the structure and in glycerophospholipid I have H2, I have R2 here, I have R1 here, I

have my phosphate and I have to this, linked an X. This is my polar part. What can happen

is if this forms R2 and for R1 I have straight chain carbon fatty acids and this is my polar

head groups. I will have a polar head group that is this part, I will have a long carbon

chain which is this part if I happen to have no double bond formation and if I happen to

have double bond formation I will bend it like this. I have specific properties of the

fatty acids that tell me that the pKas are around 4.5 to 5 making them or ionizing

them at physiological pH and I have specific melting points for these depending on the

length of the chain and on the number of the double bonds that we have here.

Because the more the number of double bonds, you are disrupting the organized structure

that it would have. What would happen if it would look perfect? This is the way they would

be organized. But if you had a kink you would have say, one that was shaped like that, one

that was disrupted like that. So what would happen to this? This would melt easier than

this. So the more the number of double bonds the more the disruption in the structure;

you would have lower melting point which is why a saturated fatty acid is solid at room

temperature.

Then we went on to study all the different types of lipids that we could have and these

are the different types of molecular arrangements of lipids that we can have and in the next

class we will see how we can organize this into an actual lipid bilayer in the protein

and we will see how proteins are embedded and how they can help in the transfer of materials

inside and outside the cell. Thank you. We continue our discussion on lipids and membranes

and in the last class we learnt what comprises lipid of the membranes. We learnt that we

can have a glycerophospholipids or a spingolipids that will look or could assemble into basically

bilayers. Now we are going to look at the properties of the lipid bilayer and see how

it forms and what are the basic physical and chemical properties of this bilayer? We will

learn about the transportation later on from one end of the cell to the other.

If you look at the properties of the lipid bilayer they are usually impermeable to polar

molecules or ions. You understand why that is so because you have the polar head groups

on the surface only and if we have to have a transportation from the inside of the cell

to the outside or from the outside of the cell to the inside then it is not possible

because of the hydrophobic chains that are present in traversing the whole membrane.

Normally the membranes are impermeable to the polar molecules or ions unless we have

some specific proteins that facilitate the transfer. We will see how we can have active

transport or passive transport. The membranes themselves are flexible but they are very

strong and they are durable. They do not just rupture. The membranes can be about 40 A thick

or even more than that and membranes are associated with specific proteins that have definite

activities. The basic properties of the bilayer are that they do not allow the transport of

the ions unless assisted by a protein. They are flexible; they are quite thick 40 A and

they are associated with proteins that have specific activity associated with it. When

we speak of the interior of the lipid bilayer it means we are speaking about the hydrophobic

tails. That is what the interior of the bilayer is. The exterior of the bilayer is the polar

portion, the polar head groups that forms the inside and the outside and the interior

is highly fluid and we will see how that fluidity occurs, why it occurs and what its usefulness

is.

In the liquid crystal state, the hydrocarbon chains of the phospholipids are disordered.

They are in constant motion because they are suspended. The cells are suspended in the

plasma. There is a cytosol to it there is an extra cellular matrix to it. So they are

all suspended. Because of their constant movement they are in constant motion and they are highly

disordered as well. polar head groups - that is the way we can bring about a change in

the curvature of the two layers by changing the size and by changing the packing. We change

the packing by changing the fatty acids. So this is what we can do. What we have here

is the cytosol. What is the cytosol? It is inside the cell.

Extra cellular space means it is outside the cell. All these are different membranes. Red

ones, the black ones and blue one with sugar attached to them are more preferred on the

surface and a few red and black ones in the inner leaflets but populated more by green

and yellow ones. We have different polar head groups. The green, the yellow, the blue and

the black of the different polar head groups and the chains are also going to be different

depending on the type of fatty acids that we have. Phosphatethyl ethanolamine that is

PE and phosphatethyl serine are usually in the inner layer. So the ethanolamine type

and the serine type are preferred in the inner layer and in the outer layer we have the sphingomyelin

and the choline type, the phosphatethyl choline. We have more of the phosphatethyl choline

and the sphingomyelin on the outside and more of the phosphatethyl ethanolamine and serine

on the inside. We have to look at membrane proteins. What are membrane proteins going

to do?

They are going to help in the transfer of ions. They are usually of three types. You

can have peripheral proteins, integral proteins or ones that have a lipid anchor. The peripheral

proteins are the ones that are marked in green here that are on the periphery of the membrane.

The ones marked in red are integral proteins that are sort of embedded in the bilayer.

The lipid anchor ones have a lipid chain attached to them that the lipid chain of which interacts

with the hydrocarbon chains of the lipid fatty acids. These are the three types of membranes

proteins that we can have and we will see the properties of the membrane proteins.

The Description of Lecture - 13 Lipids and Membranes 1