There are many instances of this phrase in the Bible (King James Version).

Exodus 20:13: Thou shalt not kill.

Deuteronomy 5:17: Thou shalt not kill.

Matthew 5:21: Ye have heard that it was said by them of old time, Thou shalt not kill; and whosoever shall kill shall be in danger of the judgment.

Romans 13:9: For this, Thou shalt not commit adultery, Thou shalt not kill, Thou shalt not steal, Thou shalt not bear false witness, Thou shalt not covet; and if there be any other commandment, it is briefly comprehended in this saying, namely, Thou shalt love thy neighbour as thyself.